| 引用本文: | 李杨子，黄华宇，贺茂勇，张婧雯，张帆，邓丽，李峥岩，
张宁，周江迪.2023.植物的非传统稳定同位素前处理及测定技术研究进展[J].地球环境学报,14(3):284-296 |
| LI Yangzi, HUANG Huayu, HE Maoyong, ZHANG Jingwen, ZHANG Fan, DENG Li, LI Zhengyan, ZHANG Ning, ZHOU Jiangdi.2023.Advances in non-traditional stable isotope pretreatment and determination techniques for plants[J].Journal of Earth Environment,14(3):284-296 |
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| 摘要: |
| 一直以来植物都是多领域的研究重点，随着非传统稳定同位素的不断发展，植物非传统稳定同位素研究日益增加。但因植物样品中有机物含量高，同位素分馏复杂，样品前处理及测定中存在诸多难题。本文对植物样品非传统稳定同位素工作中常用的前处理方法和测定技术进行了总结。综述了直接萃取法、湿法消解和高温灰化法的原理、操作和优缺点；以K、Ca、Mg、Fe、B等典型非传统稳定同位素为例介绍了非传统稳定同位素常用测定技术，旨在为植物样品的非传统稳定同位素测定进行梳理，最后对其应用方向和测试方法的发展方向进行了展望。 |
| 关键词: 植物 非传统稳定同位素 植物消解 同位素测定 |
| DOI：10.7515/JEE221016 |
| CSTR： |
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| 基金项目:中国科学院战略性先导科技专项（B类）（XDB40000000）；陕西省杰出青年科学基金（2022JC）；国家自然科学基金项目（41991252，42173012） |
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| Advances in non-traditional stable isotope pretreatment and determination techniques for plants |
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LI Yangzi, HUANG Huayu, HE Maoyong, ZHANG Jingwen, ZHANG Fan, DENG Li, LI Zhengyan, ZHANG Ning, ZHOU Jiangdi
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1. College of Urban and Environmental Science, Northwest University, Xi’an 710127, China
2. State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710061, China
3. School of Earth Science and Resources, Chang’an University, Xi’an 710054, China
4. College of Mining Engineering, Taiyuan University of Technology, Taiyuan 030024, China
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| Abstract: |
| Background, aim, and scope Plants are involved in the biogeochemical cycle of various elements and have been hot topics in the research of food safety, health care, pollution control, ecology and environment. Non-traditional stable isotopes as new techniques are being applied in plant isotope research. Due to the high organic matter content in plants, the complexity of isotopic fractionation, and the difficulties in sample processing and determination, the non-traditional stable isotope research in plants develop slowly compared with elemental research. Materials and methods This paper summarizes the pre-treatment processes and determination methods of non-traditional stable isotopes (such as K, Ca, Mg, Fe and B) in plant samples. Results Relevant studies reported non-traditional stable isotope techniques used in the fields of food identification and traceability, migration and transformation of contaminants, and ecological and environmental status. Discussion We analyzed the sample pretreatment and isotope determination methods in plants. Plant samples need to be ablated into a transparent solution for subsequent processing and determination, and the most commonly used methods are direct extraction, wet ablation and high temperature ashing. The digested samples also need to be chemically purified to separate the target elements to avoid the interference in isotope determination. The isotope determination is performed by thermal ionization mass spectrometry (TIMS), inductively coupled plasma mass spectrometer (ICP-MS) or multi-collector ICP-MS. The difference between laser ablation -ICP-MS and laser ablation -MC-ICP-MS does not require ablation and chemical separation for determining sample isotopes. Conclusions The high organic content and complex composition of plant samples make the pretreatment process more difficult and require more consideration of sample digestion conditions as well as the interference of organic matter may exist in the separation and purification and analytical determination processes. We should considerate the selection of suitable resins, exchange columns and leaching acids owing to the different properties of elements and the information on the fractionation of the isotope leaching, the residual of the resin blank and the contribution of the blank. Finally, during the mass spectrometry process, all factors affecting the determination of the target isotope should be correctly evaluated and deducted. On the other hand, an adaptive calibration method should be selected to obtain the correct results. Recommendations and perspectives There are still many potential non-traditional stable isotopes that have not yet been applied in plant-related studies. Moreover, laser exfoliation systems have a promising future in the field of isotope determination of plant samples. The advantages of simple pretreatment and the possibility of micro-regional in situ analysis will greatly increase the scope of application of non-traditional stable isotopes. |
| Key words: plant non-traditional stable isotopes plant digestion isotope determination |
