| 摘要: |
| 人体牙釉质是记录环境铅（Pb）暴露信息的较好载体。本研究采用微波消解-电感耦合等离子体质谱仪（ICP-MS）联用的方法测定来自高Pb暴露区（铅锌冶炼厂地区）和Pb暴露对照区（农业种植区）附近长期居住/不同年龄段人体离体牙的Pb含量，结果显示：高Pb暴露区离体牙表现出Pb含量高且随居民年龄增长而增高的趋势，离体牙Pb含量从6—12岁儿童的(60.8±15.3) μg∙g−1增加到65岁以上老年人的(200.1±30.5) μg∙g−1；而铅暴露对照区人体离体牙Pb含量低且基本随年龄增长变化不大。Pb主要通过呼吸道和消化道进入人体并在牙齿中富集，因此，牙齿中的Pb含量与生活环境密切相关。研究结果表明：生活在高Pb暴露区的人群，环境中的Pb可能通过各种介质（空气、水、实物等）不断进入人体，其牙齿不断蓄积环境来源的Pb。因此人体离体牙Pb有可能作为人体长期Pb暴露生物标
志物。 |
| 关键词: 铅暴露 离体牙 铅含量 年龄 生物标志物 |
| DOI：10.7515/JEE212034 |
| CSTR：32259.14.JEE212034 |
| 分类号: |
| 基金项目:陕西省自然科学基础研究计划项目（2020JQ-875）；国家自然科学基金项目（42003016）；2022年大学生创新创业训练计划项目（121522001） |
| 英文基金项目:Natural Science Basic Research Plan in Shaanxi Province of China (2020JQ-875); National Natural Science Foundation of China (42003016); 2022 Student Innovation and Entrepreneurship Training Program (121522001) |
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| Relationship between lead content in isolated human teeth and human age in different lead exposure areas and its significance |
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LI Zixia, LI Xiangzhong, LU Hai, ZHOU Fang, ZHANG Haoqi
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1. Department of Stomatology, Xi’an Medical University, Xi’an 710021, China
2. Research Center for Earth System Science, Yunnan University, Kunming 650500, China
3. Division of Chemical Metrology & Analytical Science, National Institute of Metrology, China, Beijing 100013, China
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| Abstract: |
| Background, aim, and scope Heavy metal lead in human dental enamel is an archival record that can record information about environmental Pb exposure. This study analyzed the Pb content of isolated teeth from residents with different ages living in different lead exposure areas by inductively coupled plasma mass spectrometry (ICP-MS). The purpose is to discuss the characteristics and age relationship of Pb content in human teeth from different Pb exposure areas. Materials and methods The people living in different lead exposure areas, namely, lead-zinc smelter areas (high lead exposure areas) and agricultural planting areas (low lead exposure areas), were selected. Isolated human teeth from these two different lead exposure areas were collected. The isolated human teeth were dissolved via microwave digestion, and the Pb contents of the isolated human teeth after microwave digestion were analyzed using ICP-MS. Results The lead content in the teeth of people who lived in the high lead exposure area increased with age. The lead content in the teeth increased from (60.8±15.3) μg·g−1 in children aged 6—12 to (200.1±30.5) μg·g−1 in adults over than 65. However, the lead content in the teeth of people who lived in the low lead exposure areas did not increase much with age. Discussion Pb enters the human body through the respiratory tract and digestive tract. The level of Pb in teeth is related to various factors, such as the living environment and dietary habits. Residents in high Pb exposure areas were affected by environmental Pb. In areas where Pb concentrations are high, the Pb content in the teeth of residents is also high. In addition, the content of Pb in their teeth gradually increases with age. Conclusions The content of heavy metals in people living in the high lead exposure areas was higher than that in the low lead exposure areas. The lead content of teeth records the cumulative exposure to lead in the environment. Recommendations and perspectives The results are expected to provide an important scientific basis for the use of the lead content of teeth used as a biomarker for long-term lead exposure in humans. |
| Key words: lead exposure isolated human teeth lead content age biomarkers |
